PhD Student: Decoding Epigenetic Mechanisms of Gene Regulation in T Cells

The group of Fred van Leeuwen is looking for a PhD candidate to join our team working on mechanisms and functions of epigenetic pathways in T cells. In this project, you will study how targeting an epigenetic writer protein called DOT1L reprograms cytotoxic T cells. T cells are crucial players in anti-tumor responses, but tuning their activity remains a challenge. By understanding the epigenetic mechanisms by which DOT1L works, we hope to ultimately strengthen T cells so they can fight cancer cells more effectively. Do you want to join us in uncovering new insights into T-cell biology and help pave the way for more effective cancer immunotherapies? Take this opportunity to grow as a scientist while contributing to research that could make a real difference for cancer patients.

In this research project you will:

• Apply innovative and complementary strategies in a tractable human T cell model, including CRISPR-Cas9 based genome engineering and epigenetic editing, epigenomics and transcriptomics, and unique DNA-barcode-based epigenetic screens in yeast.

• Validate mechanisms in primary human CD8 T cells.

• Gain molecular insights to provide leads for improved epigenetic perturbation of CD8 T cells to enhance cytotoxic activity towards cancer cells.

Our group is part of the Division of Gene regulation, which focuses on (epigenetic) mechanisms of genome-organization and gene transcription. We work in close collaboration with the Division of Molecular Oncology and Immunology for complementary expertise on immune cell biology, genetic modification and phenotyping of T cells, and T cell therapy. Our research at the NKI is supported by state-of-the art core facilities, including imaging, flow cytometry, proteomics, genomics, screening, and many more. More information about the Van Leeuwen lab and our recent work on T cells can be found here: www.nki.nl/research/research-groups/fred-van-leeuwen/.

Why the Netherlands Cancer Institute?

Science is at the heart of everything we do at the NKI. But breakthroughs don't happen through science alone; they happen through people – curious minds, bold thinkers, people who challenge assumptions and turn complex questions into meaningful discoveries. Around 600 scientists, together with 150 scientific and 150 administrative professionals, work here across the full spectrum of cancer research, from fundamental science to clinical innovation. With roughly 55 nationalities represented, we form a vibrant international community where English is our shared language and every voice carries equal weight. Our institute sits just 15 minutes by bike from the heart of Amsterdam.

We are looking for a motivated and collaborative PhD candidate who is able to work independently and approaches challenges with a proactive, problem-solving mindset. You enjoy working with others and have excellent social and communication skills, enabling you to contribute effectively to an international and multidisciplinary team. In addition, you have:

• You hold a MSc degree in (molecular) biology or a related discipline (e.g. medical biology, cancer biology, genetics);

• You have demonstrable experience with basic molecular biology techniques;

• You have affinity for (or ideally experience with) computational biology;

• You are fluent in academic English, both spoken and written;

• Prior experience (e.g. internship) in the field of gene regulation / chromatin biology is an asset;

• You are interested in fundamental epigenetic mechanisms in the context of cancer immunology.

Your development opportunities and employment conditions

As a PhD candidate at the Antoni van Leeuwenhoek, you will work in a cutting-edge, dynamic and international research environment, surrounded by experts from a wide range of scientific disciplines. You will have ample opportunities to collaborate across disciplines and further develop your scientific, personal and professional skills. Throughout your PhD trajectory, you will receive training and supervision through the Oncology Graduate School Amsterdam (OOA), supporting you in both your research and your personal development.
You will also be represented by an active PhD Council, ensuring that the perspectives and interests of PhD candidates are heard. Joining our comprehensive cancer center offers you an inspiring environment in which research and patient care come together, providing a unique opportunity to learn, grow and contribute to advancing cancer research and care. The basis for your employment conditions is in accordance with the CLA Hospitals. You will receive from us:

• A temporary contract for a period of four years, for 36 hours per week;

• A gross monthly salary between €3.813,- and €4.629,-, based on a 36-hour working week, in line with the OIO scale and depending on your experience;

• 144 holiday hours and 57 hours of Personal Life Budget, with full-time employment;

• Annual holiday pay of 8.33% and a fixed end-of-year bonus of 8.33%;

• A travel allowance of €0.23 per km;

• Free parking at the Netherlands Cancer Institute. And with our bicycle plan and discount on public transport we also make it attractive for you to leave the car at home;

• A great opportunity in a specialised hospital where you can also continue to learn and grow yourself if you wish: the AVL Academy offers innovative and inspiring education in the field of oncology professional knowledge, skills and personal development;

• Opportunity to exercise at our Fit Boutiqs;

Interested?

We are curious about your talent! Will you be the one to make a difference in the world of cancer care and research? Apply by the 'Apply now' button at the bottom of the page. Please include in your application:

• Your application letter, explaining why you are excited about this PhD position, and what experience and personal qualities make you a strong candidate.

• A CV, including your grades. If you have no MSc certificate yet, explain briefly what you still need to complete and provide your expected graduation date.

• Contact information of 2 references. Provide name, email address, phone number (do not submit reference letters).

Ensure that your references information and an overview of your grades are attached together with your application letter and CV, as there is no option to submit them separately. Take note: please respond to this vacancy only via the application button, and not via email.

This vacancy is open for applications until the 1st of October. We assess applications on an ongoing basis, so we encourage you to apply as soon as possible.

For further information about the position please contact Fred van Leeuwen, Group Leader, via fred.v.leeuwen@nki.nl.

For questions about the application procedure, please contact Pascal van Kippersluis, Recruiter, via p.v.kippersluis@nki.nl.